Common cell biology experiments

中文名称 英文 编 号
1.细胞培养 Cell culture
1.原代细胞分离 Mitochondrial isolation
Cell collection or isolation
3.细胞增殖检测(Cell viability) Cell counting
Growth rate analysis
MTT Assay
CCK-8
BrdU Assay
WST-1 assay
Colony formation assay
CyQuant method
Quick Cell Proliferation Assay Kit (ABCAM)
The Cell Titer 96 Aqueous One Solution cell proliferation assay
EdU assay
4.细胞周期检测:流式检测细胞周期,标记有丝分裂百分率法 Cell cycle assay
5.细胞转移能力检测 Transwell assay
Cell invasion assay
Wound healing assay
6.细胞毒性检测 Lactate dehydrogenase (LDH) release detection of cells
7.细胞凋亡\焦亡检测 Annexin V-FITC/PI staining(Flow cytometry)
Mitochondrial membrane potential measurements(ΔΨm)
Mitochondrial swelling
Caspase activity assay
Scanning Electron microscopy
The Cell Death Detection ELISA Kit
Live-cell imaging
TUNEL
Measurement of SOD or reactive oxygen species(ROS)
8. 细胞恶性程度 Anchorage-independent growth in soft agar
9.细胞屏障 Transepithelial electrical resistance (TEER)
Alterations in the fluorescence of propidium iodide (PI)
Cell permeabilization
Paracellular permeability
10.细胞代谢 ATP and ADP determination
Mitochondrial complex activity analysis
Cytochrome C reduction level
K+ transport
Ca2+ uptake assays
intramitochondrial pH (pHin)
Cellular and mitochondrial respiration(OCR)
Cell metabolism activity assay
Spectrophotometric analyses ofrespiratory chain enzymes
Seahorse Extracellular Flux Analyzer
Electrophysiological measurements
Extraction and quantification of coenzyme Q
NADP+/NADPH
Mitochondrial membrane fluidity
11.核酸分析 Single nucleotide polymorphism (SNP)
qRT-PCR
DNA extraction
RNA extraction and cDNA synthesis
12.免疫细胞化学 Immunohistochemistry staining
13.分子互作 Luciferase reporter assay or Immunofluorescence microscopy analysis
14.细胞转染 Transfection procedure
15.转运分析 Transport assays
16.蛋白表达 Western blot
ELISA
17.细胞黏附实验 Cell Adhesion Assay
18.细胞共培养实验 Co-culture
19.流式细胞技术 Flow cytometry
脂质过氧化 Lipid extraction and peroxidation
信号通路 mTOR regulators treatment
氧化应激 Oxidative stress(MDA) and Oxidative damage (HPs,GPX,GR,H2O2)
细胞表面作用力 Atomic force microscopy
人工膜泡 Liposome preparation
心肌 Measurement of cytosolic basal Na+ fluorescence intensity
质谱 MALDI-TOF mass spectrometry
酵母胞浆 Analysis ofCCCP-induced acidification of yeast cytosol
电生理 whole-cell configuration (patch-clamp technique) record ionic currents
脱颗粒实验 Degranulation assays
中文名称 1.细胞培养 1.原代细胞分离 3.细胞增殖检测(Cell viability) 4.细胞周期检测:流式检测细胞周期,标记有丝分裂百分率法 5.细胞转移能力检测 6.细胞毒性检测 7.细胞凋亡\焦亡检测 8. 细胞恶性程度 9.细胞屏障 10.细胞代谢 11.核酸分析 12.免疫细胞化学 13.分子互作 14.细胞转染 15.转运分析 16.蛋白表达 17.细胞黏附实验 18.细胞共培养实验 19.流式细胞技术 脂质过氧化 信号通路 氧化应激 细胞表面作用力 人工膜泡 心肌 质谱 酵母胞浆 电生理 脱颗粒实验
英文 编 号 Cell culture Mitochondrial isolation Cell collection or isolation Cell counting Growth rate analysis MTT Assay CCK-8 BrdU Assay WST-1 assay Colony formation assay CyQuant method Quick Cell Proliferation Assay Kit (ABCAM) The Cell Titer 96 Aqueous One Solution cell proliferation assay EdU assay Cell cycle assay Transwell assay Cell invasion assay Wound healing assay Lactate dehydrogenase (LDH) release detection of cells Annexin V-FITC/PI staining(Flow cytometry) Mitochondrial membrane potential measurements(ΔΨm) Mitochondrial swelling Caspase activity assay Scanning Electron microscopy The Cell Death Detection ELISA Kit Live-cell imaging TUNEL Measurement of SOD or reactive oxygen species(ROS) Anchorage-independent growth in soft agar Transepithelial electrical resistance (TEER) Alterations in the fluorescence of propidium iodide (PI) Cell permeabilization Paracellular permeability ATP and ADP determination Mitochondrial complex activity analysis Cytochrome C reduction level K+ transport Ca2+ uptake assays intramitochondrial pH (pHin) Cellular and mitochondrial respiration(OCR) Cell metabolism activity assay Spectrophotometric analyses ofrespiratory chain enzymes Seahorse Extracellular Flux Analyzer Electrophysiological measurements Extraction and quantification of coenzyme Q NADP+/NADPH Mitochondrial membrane fluidity Single nucleotide polymorphism (SNP) qRT-PCR DNA extraction RNA extraction and cDNA synthesis Immunohistochemistry staining Luciferase reporter assay or Immunofluorescence microscopy analysis Transfection procedure Transport assays Western blot ELISA Cell Adhesion Assay Co-culture Flow cytometry Lipid extraction and peroxidation mTOR regulators treatment Oxidative stress(MDA) and Oxidative damage (HPs,GPX,GR,H2O2) Atomic force microscopy Liposome preparation Measurement of cytosolic basal Na+ fluorescence intensity MALDI-TOF mass spectrometry Analysis ofCCCP-induced acidification of yeast cytosol whole-cell configuration (patch-clamp technique) record ionic currents Degranulation assays

Bib:

期刊名:Journal of bioenergetics and biomembranes 备注\编号
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